Insertion junction: LMJ.RY0402.057065_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.057065
Chromosome:chromosome 7
Location:4118147
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g342850 ODA5,DCC3 (1 of 2) PTHR21694//PTHR21694:SF18 - UNCHARACTERIZED // COILED-COIL DOMAIN-CONTAINING PROTEIN 114; Outer Arm Dynein assembly protein 5 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAATCCAACCTCGACACTGAACGCCACC
Internal bar code:GTTGTTTACTCAGTATCAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:99.2105
LEAP-Seq n confirming:11687
LEAP-Seq n nonconfirming:93
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCGTTCTTCCTCGCTTC
Suggested primer 2:CGGTGTAAGCAAGCAGTTGA