| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057088 |
| Chromosome: | chromosome 9 |
| Location: | 7531078 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414200 | VPS26 | (1 of 1) K18466 - vacuolar protein sorting-associated protein 26 (VPS26); Subunit of Retromer complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACACGAACAGATCATGTGCTGTTGGG |
| Internal bar code: | GTCAAGTGTCGGTCTGCGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 949 |
| LEAP-Seq percent confirming: | 99.8308 |
| LEAP-Seq n confirming: | 590 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | TCGTCTGCCATTCTCAAGTG |