| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057140 |
| Chromosome: | chromosome 2 |
| Location: | 3815272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095550 | (1 of 1) PTHR14187//PTHR14187:SF51//PTHR22908 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED // MIDASIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATCATTGTGACAGGAAATAGATGACCG |
| Internal bar code: | CCCCTTACAAGGGGTCATAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 93.3668 |
| LEAP-Seq n confirming: | 929 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACTTGTCCAGGTCAGC |
| Suggested primer 2: | AGGTGCGTTCATCTAGCGTT |