| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057160 |
| Chromosome: | chromosome 14 |
| Location: | 4031672 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633500 | (1 of 1) IPR024224 - DENND6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGCGGCCGACTCAGCCGCGAGCACCC |
| Internal bar code: | TGATAGGACTCGAAGCGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 98.4899 |
| LEAP-Seq n confirming: | 587 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAACTGGAGACTGGAGG |
| Suggested primer 2: | CACACACACACACACACGGT |