Insertion junction: LMJ.RY0402.057217_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.057217
Chromosome:chromosome 7
Location:161394
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g313250 (1 of 1) 3.6.3.17//3.6.3.25 - Monosaccharide-transporting ATPase // Sulfate-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCATATCACAGCGAGTACCCAACTCTC
Internal bar code:TTTTAACAACGAATGTCATGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:99.3377
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTGGACCTACATGGGCT
Suggested primer 2:GGTGTCATGTGACCAGTTGC