Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.057220 |
Chromosome: | chromosome_13 |
Location: | 3357182 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g586750 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCTTCTCTACCTGCTGTCGGCACTCGCTG |
Internal bar code: | CAGATCGCGTAGCCGTCTCGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 907 |
LEAP-Seq percent confirming: | 99.7013 |
LEAP-Seq n confirming: | 7678 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAAGAGCAATGCTGTGGAA |
Suggested primer 2: | TAGAGGTGCAGTGTCGATGC |