| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057227 |
| Chromosome: | chromosome 5 |
| Location: | 705783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGCGCCACGGTTCGGGAGATCGGTGCA |
| Internal bar code: | TACCACGCATGCGCATACGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 93.8849 |
| LEAP-Seq n confirming: | 1566 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATGCACTTTGCAGGAGA |
| Suggested primer 2: | AATGGCTTCGTTTGGTTGAC |