| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057227 |
| Chromosome: | chromosome 13 |
| Location: | 4437479 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603350 | CYG70 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAAGTGCCCCCCCGACGAGCCCGTGCGT |
| Internal bar code: | GGCGCCACTGGTCGGCTCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 98.4094 |
| LEAP-Seq n confirming: | 20540 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCGTGGCTATAGTGGT |
| Suggested primer 2: | CTGAACAAATCTCTCCCCCA |