| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057234 |
| Chromosome: | chromosome 6 |
| Location: | 8886591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311000 | FBT2 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCACCAGGCTGCCGCTGAGCGCCGCGGT |
| Internal bar code: | TTGGGTCACGCCATGCAATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 99.9001 |
| LEAP-Seq n confirming: | 3001 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTGTCTCTGTTCCTG |
| Suggested primer 2: | GAGGTGATGGTGGTGAAGGT |