Insertion junction: LMJ.RY0402.057245_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057245
Chromosome:chromosome 16
Location:1752603
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCGCCGGACACGGCAACTCCGCGCAGGT
Internal bar code:CCGCATAATGCTCGACCATTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:216
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:175
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCGTCCAGTACGGCAATC
Suggested primer 2:AACAACCCTGTATGCCTTGC