Insertion junction: LMJ.RY0402.057246_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.057246
Chromosome:chromosome 7
Location:4109474
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g342920 (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACGGCAGCCCGATGGCCCCACGGCACG
Internal bar code:AAATATTCTACAATTTGGAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:99.7573
LEAP-Seq n confirming:411
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACCTGTCCGTGTTTC
Suggested primer 2:AAACTGAATCGAACCCAACG