| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057250 |
| Chromosome: | chromosome 9 |
| Location: | 2422192 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391500 | ATG9 | (1 of 1) K17907 - autophagy-related protein 9 (ATG9); Autophagy related | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTACCTCCGTGGTTTTGTGACGCGGCAG |
| Internal bar code: | GCGGCTAGAAAAACAATACCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.5864 |
| LEAP-Seq n confirming: | 10834 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAACACATGCGTAATC |
| Suggested primer 2: | GTAGCGTGTCCGTTACCGTT |