Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.057262 |
Chromosome: | chromosome_1 |
Location: | 6933427 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g049950 | VLE1,SPO | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGTGCGCCGGCAGCGGGGACACCCCACC |
Internal bar code: | CCGTAACGACTACGTCGGGACT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1226 |
LEAP-Seq percent confirming: | 99.7604 |
LEAP-Seq n confirming: | 61615 |
LEAP-Seq n nonconfirming: | 148 |
LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTTTCCAAGTGCACTCCTC |
Suggested primer 2: | CGAAGTTGAAGTCAGGCACA |