Insertion junction: LMJ.RY0402.057264_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.057264
Chromosome:scaffold 24
Location:64421
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGCCATGTCCAGTGCGTTCTCAAACAC
Internal bar code:TCATAGGGTCGTTCGACAGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:264
LEAP-Seq percent confirming:99.5881
LEAP-Seq n confirming:7011
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAGGGGTAGGAGGTATG
Suggested primer 2:CACTCCTACCCTCCTCCCTC