| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057283 |
| Chromosome: | chromosome 16 |
| Location: | 2839545 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663350 | CLPX1,CLPX | ClpX chaperone; (1 of 1) K03544 - ATP-dependent Clp protease ATP-binding subunit ClpX (clpX, CLPX) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGGCCGGCGTAACGGACGCCTTCTCG |
| Internal bar code: | TTTCGATTATTCAGTCCTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 99.712 |
| LEAP-Seq n confirming: | 1731 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTATCTGAGGCTGCCGT |
| Suggested primer 2: | GACCGGTAGCCCTAGACTCC |