| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057293 |
| Chromosome: | chromosome 12 |
| Location: | 2679750 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504000 | (1 of 2) PTHR12121:SF34 - LD39302P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGTGCGCGGCCTCGCACTGTTTGTGC |
| Internal bar code: | TCATTGTAGTCCGCCACGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 98.2595 |
| LEAP-Seq n confirming: | 3218 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTAGCCAAGCAGTGTA |
| Suggested primer 2: | ACCCAATCACAGCTACCGAC |