| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057313 |
| Chromosome: | chromosome 7 |
| Location: | 2544525 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329850 | SNRK2P,KCN5 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCGACGCTGCAGACGAAACCAGGCACC |
| Internal bar code: | TGATCGTACATACACGCCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.6678 |
| LEAP-Seq n confirming: | 300 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGGCTTCCAGATTGTC |
| Suggested primer 2: | CTTGCCTCTGCTAGGTGTCC |