| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057334 |
| Chromosome: | chromosome 12 |
| Location: | 1032524 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491250 | (1 of 1) IPR003615//IPR010995 - HNH nuclease // DNA repair Rad51/transcription factor NusA, alpha-helical | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCATAGGACCTCAGCTGACCAAGGCACA |
| Internal bar code: | GGGCGCGTCGATTGCTAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1082 |
| LEAP-Seq percent confirming: | 99.7159 |
| LEAP-Seq n confirming: | 2457 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGGCTGTATTATGCCAG |
| Suggested primer 2: | CCTTGTTGTGACCGGTTTCT |