Insertion junction: LMJ.RY0402.057340_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.057340
Chromosome:chromosome 6
Location:6327775
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g291850 (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTCTCGACTGCTCGCGGCTGCGCCTTA
Internal bar code:TGATATGTTGTTCGGGGAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:55.7072
LEAP-Seq n confirming:898
LEAP-Seq n nonconfirming:714
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGATCCTGCAGCACTACGAC
Suggested primer 2:GTGTACACCACCAGCGATTG