| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057352 |
| Chromosome: | chromosome 12 |
| Location: | 1876276 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511400 | CYCR3,PPR4 | PentatricoPeptide Repeat protein 4; (1 of 1) PTHR10177//PTHR24015 - CYCLINE // FAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAAAAGCATGTGCGCGGGGCGGCACGG |
| Internal bar code: | AGGTATTGTCGCGTGGGGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 99.56 |
| LEAP-Seq n confirming: | 1810 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCAACAATTACGGCTCT |
| Suggested primer 2: | AATTCCACGTAACAGCCCAG |