| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057362 |
| Chromosome: | chromosome 12 |
| Location: | 1559787 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACAGCCCATCCTCGTCAGCGGAATGCC |
| Internal bar code: | TCGCATCTTACATTCGAGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 99.7335 |
| LEAP-Seq n confirming: | 4117 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTATACCTGCTTGCTTG |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |