Insertion junction: LMJ.RY0402.057409_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.057409
Chromosome:chromosome 11
Location:3729650
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g482900 RAB20,RAB18C,RABC3 Small Rab-related GTPase; (1 of 3) K07910 - Ras-related protein Rab-18 (RAB18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAACAGCGGAGCGGGCAAGCAACCCGCCT
Internal bar code:TGATACTCGACGGGCCATTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:620
LEAP-Seq percent confirming:99.4307
LEAP-Seq n confirming:3144
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCATTTGACTTTGGACTT
Suggested primer 2:CCTCGGGCTAACGTACACAT