Insertion junction: LMJ.RY0402.057416_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.057416
Chromosome:chromosome 1
Location:2468524
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g013900 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCTCCCGACCCTGCGGTTCCCCACCCT
Internal bar code:AGCGCGAAACCCGTCTTTTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1038
LEAP-Seq percent confirming:98.9418
LEAP-Seq n confirming:935
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCATCACAACACAATCC
Suggested primer 2:TTCGTGTGTAGCTACGGTGC