| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057419 |
| Chromosome: | chromosome 3 |
| Location: | 6050671 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191300 | LCI34,LCI35 | Low-CO2-inducible protein 35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCTAGACATGAGCTGACAAGCCCCAGC |
| Internal bar code: | GGGTCCTCAGGACGGAAGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.0541 |
| LEAP-Seq n confirming: | 733 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATTGGGTCAGCCTGCACT |
| Suggested primer 2: | TGAGACGTACGTGCCATCAT |