| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057426 |
| Chromosome: | chromosome 10 |
| Location: | 6515919 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGTAATATTCAGGAGCGCGCCCTGCG |
| Internal bar code: | GGGCAGCGTCGAGAATAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 98.017 |
| LEAP-Seq n confirming: | 346 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACGACTTACTGGTCAGA |
| Suggested primer 2: | TGTTGTAGCGAACCAAGCAG |