| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057435 |
| Chromosome: | chromosome 3 |
| Location: | 5208750 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182700 | (1 of 1) PTHR31832//PTHR31832:SF3 - FAMILY NOT NAMED // B-BOX ZINC FINGER PROTEIN 20 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCATTGCAAGTCCACATTTGTTCCCG |
| Internal bar code: | GCCGGCGCCATGAGTAAAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 99.8214 |
| LEAP-Seq n confirming: | 1118 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGATACTCGTTTCGTGA |
| Suggested primer 2: | CTCGAAGTTGAAGAGTGCCC |