| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057437 |
| Chromosome: | chromosome 17 |
| Location: | 5949910 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGAGGGAGGGAGCGCGTGAGGCAGGTA |
| Internal bar code: | CGGACCAAATTCAGCTCGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 60.5563 |
| LEAP-Seq n confirming: | 1437 |
| LEAP-Seq n nonconfirming: | 936 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTACAGCCACCTCTCTGC |
| Suggested primer 2: | GCAAGAAGTGAAGGCGTAGG |