| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.057503 | 
| Chromosome: | chromosome 17 | 
| Location: | 6613889 | 
| Confidence (%): | 73 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre17.g743647 | (1 of 32) IPR029062 - Class I glutamine amidotransferase-like | 3'UTR | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCATCGCCCACCCCGCCTGAAACCTCCT | 
| Internal bar code: | CGGGATCGGCAACAGCAGCTGA | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 | 
| LEAP-Seq percent confirming: | 97.6942 | 
| LEAP-Seq n confirming: | 805 | 
| LEAP-Seq n nonconfirming: | 19 | 
| LEAP-Seq n unique pos: | 8 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCAATCTGTCACTGGC | 
| Suggested primer 2: | ACTTTGGTTAGGCATCACGG |