| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057528 |
| Chromosome: | chromosome 9 |
| Location: | 1221453 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399916 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCGCTAGTCGGCAGGCGCGTGAGTGG |
| Internal bar code: | TCGCATTTCGGTATTGTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 350 |
| LEAP-Seq percent confirming: | 99.1912 |
| LEAP-Seq n confirming: | 2085 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGTCGCAGTAATGTGCT |
| Suggested primer 2: | GGGAATCATCGGGGATAACT |