Insertion junction: LMJ.RY0402.057532_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.057532
Chromosome:chromosome 2
Location:5754788
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g110500 (1 of 10) IPR004087//IPR004088 - K Homology domain // K Homology domain, type 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTGTCGGCGGCGGGCGTTGTCGACAAC
Internal bar code:GACTGCAAGAGGGCCTTGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1172
LEAP-Seq percent confirming:80.2624
LEAP-Seq n confirming:7218
LEAP-Seq n nonconfirming:1775
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCACATGTTCACAAACC
Suggested primer 2:TAGTTGCTCAACCCATTCCC