Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.057551 |
Chromosome: | chromosome_9 |
Location: | 4183183 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g394065 | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCAATGTCCAGGGTGCAGGCCTTGAACTA |
Internal bar code: | TGGTATTCATAGTTGTCTCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1177 |
LEAP-Seq percent confirming: | 99.6618 |
LEAP-Seq n confirming: | 16500 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTTGTCTGGACTCTGCTG |
Suggested primer 2: | CAGCAGAACGTGGTGAGTGT |