| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057551 |
| Chromosome: | chromosome 9 |
| Location: | 4183183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394065 | FAP414 | (1 of 2) K18635 - protein SPIRAL1 and related proteins (SPR1); Flagellar Associated Protein 414 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAATGTCCAGGGTGCAGGCCTTGAACTA |
| Internal bar code: | TGGTATTCATAGTTGTCTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 99.6618 |
| LEAP-Seq n confirming: | 16500 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTGTCTGGACTCTGCTG |
| Suggested primer 2: | CAGCAGAACGTGGTGAGTGT |