| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057568 |
| Chromosome: | chromosome 12 |
| Location: | 1534798 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487000 | CSV8 | Chlamydomonas-specific family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGGGGCTGTGAACAACCGGTCGGGGG |
| Internal bar code: | TACGTATGAGCGCGGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 90.4589 |
| LEAP-Seq n confirming: | 4494 |
| LEAP-Seq n nonconfirming: | 474 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCCGTAAACGATGTTGT |
| Suggested primer 2: | ACAAGATATACGGCCGAACG |