| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057576 |
| Chromosome: | chromosome 6 |
| Location: | 3791575 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278163 | ARG9 | (1 of 1) 2.6.1.11 - Acetylornithine transaminase / Succinylornithine aminotransferase; Acetylornithine aminotransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCCCATCGCACGCTCACGTGCCCGAAC |
| Internal bar code: | AGGTCCGCAGACTCAATTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.8502 |
| LEAP-Seq n confirming: | 2666 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATGTCGATGTACTCCT |
| Suggested primer 2: | TTCGATGCAACATTTACGGA |