| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057598 |
| Chromosome: | chromosome 3 |
| Location: | 2256770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157800 | TRXL1,TRL6,TRX2 | (1 of 1) PTHR10438:SF227 - THIOREDOXIN-LIKE 2-1, CHLOROPLASTIC-RELATED; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCTCCGACTCGGTGGACGCCAGCCG |
| Internal bar code: | TTTAGTTTGTGCATAGATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.3215 |
| LEAP-Seq n confirming: | 3074 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGTGCTCTTCCTCAAAG |
| Suggested primer 2: | CCTAGACCCTCCACTCCTCC |