| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057607 |
| Chromosome: | chromosome 5 |
| Location: | 2097846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234638 | (1 of 1) 2.4.2.14 - Amidophosphoribosyltransferase / Phosphoribosyldiphosphate 5-amidotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCTCCCTCCCTCTTGGGCGGCGATTC |
| Internal bar code: | AGCGGGTGCAGGAATCCTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 630 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAATGTAACCCCTTCCCC |
| Suggested primer 2: | GTGTTGGCACAGGATGTCAC |