Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.057613 |
Chromosome: | chromosome 17 |
Location: | 184870 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g697450 | (1 of 1) K15029 - translation initiation factor 3 subunit L (EIF3L) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCCTATGTTTCCCACCTCATCGTGCCC |
Internal bar code: | CTGTAGGCCTGGTGTTCTCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 661 |
LEAP-Seq percent confirming: | 99.5122 |
LEAP-Seq n confirming: | 612 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGAGTGAGCATCACAGCG |
Suggested primer 2: | ACTTTCACCGTGTCTCCACC |