Insertion junction: LMJ.RY0402.057626_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057626
Chromosome:chromosome 6
Location:2943479
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g272850 uL10m,MRPL10,PRPL10 (1 of 1) K02864 - large subunit ribosomal protein L10 (RP-L10, MRPL10, rplJ); Mitochondrial/chloroplast ribosomal protein L10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGGCGACTGAGCGGGTGACGAAACCCC
Internal bar code:TTACGAGAGCAAGGCTTGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1071
LEAP-Seq percent confirming:98.6359
LEAP-Seq n confirming:940
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTTCTTCGAGTTGGGAC
Suggested primer 2:CGAACGGCTCTTTATGCTTC