Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.057630 |
Chromosome: | chromosome_17 |
Location: | 3362544 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g723600 | IFT81 | Intraflagellar transport protein 81 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCATATATACAAGCTTGCTCGCTGACTCC |
Internal bar code: | CCGAAGATAAAATAGGGAGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 997 |
LEAP-Seq percent confirming: | 99.1365 |
LEAP-Seq n confirming: | 8151 |
LEAP-Seq n nonconfirming: | 71 |
LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGTCCTTAAAGTCTGCCG |
Suggested primer 2: | CGCGAGATTAAGGACACACA |