| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.057630 |
| Chromosome: | chromosome_17 |
| Location: | 3362544 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g723600 | IFT81 | Intraflagellar transport protein 81 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCACCCCAAGTGTAACACAAGGACTGTTT |
| Internal bar code: | CGCCAGGTGATGGAATCAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 99.6344 |
| LEAP-Seq n confirming: | 7086 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGAGATTAAGGACACACA |
| Suggested primer 2: | CGAGTCCTTAAAGTCTGCCG |