| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.057675 |
| Chromosome: | chromosome 3 |
| Location: | 8231210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199650 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTCAAGGCAGGTTGGTGAGGCCCCCAC |
| Internal bar code: | CCGCCACTAGGCCCAGAAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 97.5904 |
| LEAP-Seq n confirming: | 243 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTCCCAGTATCCTCAA |
| Suggested primer 2: | TGTCATCTCCAGCAGACCAG |