| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057735 |
| Chromosome: | chromosome 8 |
| Location: | 4583600 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383050 | (1 of 1) 3.7.1.14 - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGGGACATCGAGGTGCATGGCAAGAC |
| Internal bar code: | CGGATTAGGCGCCAAGCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 98.659 |
| LEAP-Seq n confirming: | 515 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | CCCCTCTCAAACCACACACT |