| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057767 |
| Chromosome: | chromosome 5 |
| Location: | 701016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230804 | SNF2 | SNF2-related ATPase; (1 of 2) PTHR10799:SF573 - TRANSCRIPTION TERMINATION FACTOR 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAGATGATGTGCGCGACGCGGTTATCC |
| Internal bar code: | GAGTCTGTGTTCCCTCGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 770 |
| LEAP-Seq percent confirming: | 99.7276 |
| LEAP-Seq n confirming: | 32949 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAAGACAGGAAGCACAA |
| Suggested primer 2: | ATCTCCAAACCAAGGCACAC |