Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.057793 |
Chromosome: | chromosome 11 |
Location: | 2829835 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g477500 | (1 of 1) PF13664 - Domain of unknown function (DUF4149) (DUF4149) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGATGTTGATGTGCACATTGCTACAGG |
Internal bar code: | CGTCGGTACCATTCACTTCGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 226 |
LEAP-Seq percent confirming: | 99.7333 |
LEAP-Seq n confirming: | 374 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAACATAGGTTTTGGCGACG |
Suggested primer 2: | CCCATACATGAGCACGTCAC |