| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057803 |
| Chromosome: | chromosome 16 |
| Location: | 5029721 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684600 | SEC5 | (1 of 1) K17637 - exocyst complex component 2 (EXOC2, SEC5); Component of the Exocyst Complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGACGGTGCTGCCACGACGGCTGGA |
| Internal bar code: | AGTATATGGAATATCTCTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.6111 |
| LEAP-Seq n confirming: | 10501 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAATAAGGGAACGTGACCG |
| Suggested primer 2: | GCTCGTGGTAGATGGAGAGG |