Insertion junction: LMJ.RY0402.057816_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057816
Chromosome:chromosome 8
Location:4912551
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g385050 MAPK2 Mitogen-Activated Protein Kinase Homolog 2; (1 of 3) K04371 - mitogen-activated protein kinase 1/3 (MAPK1_3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCCCCCGCACTTTTTGTTACATCGCG
Internal bar code:ATAGGCGATCGCTTCGGGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:382
LEAP-Seq percent confirming:98.6755
LEAP-Seq n confirming:298
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTGGCTGATGGTGTATG
Suggested primer 2:AAGGCTGCTCATTGCTCATT