Insertion junction: LMJ.RY0402.057825_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.057825
Chromosome:chromosome 17
Location:2154751
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g712850 TRX23,TRL9 (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACACAGCGCAGAGGTACCGTGATACCAA
Internal bar code:GGACGTCGCACCGAAGTCTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:97.624
LEAP-Seq n confirming:945
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACGGTACCCAAGCCATA
Suggested primer 2:GCAAGCCCATCTAAGACAGC