| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057828 |
| Chromosome: | chromosome_5 |
| Location: | 3213888 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g239850 | PHO4,PHOD | Alkaline phosphatase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAACAGAAGACCTACCTAGCTAGGGGTAGC |
| Internal bar code: | TGACGCGGAAAGATCGGGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 693 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCAAACACACTAACGGC |
| Suggested primer 2: | TCGCACCTATCTCACACTCG |