Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.057834 |
Chromosome: | chromosome 7 |
Location: | 2513088 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g329600 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCAACGGCCCGGAAGACCACAGGGGG |
Internal bar code: | GTCGCTTCTCAATGGTCACGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 545 |
LEAP-Seq percent confirming: | 99.7329 |
LEAP-Seq n confirming: | 4481 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGCTACTGCAGGGTAAGGC |
Suggested primer 2: | GAAACAAGAAGCAACCCTGC |