| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.057863 |
| Chromosome: | chromosome 6 |
| Location: | 1560070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260300 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGTGGATCGTTTCATGGCACACACTCT |
| Internal bar code: | GTCAGCTTGGTGCACATACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.7105 |
| LEAP-Seq n confirming: | 2220 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTCAAGTTGGTCTTCCC |
| Suggested primer 2: | CAGGATTCACTCGTTCAGCA |