| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.057892 |
| Chromosome: | chromosome 12 |
| Location: | 5908053 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534100 | COL,CON2 | (1 of 3) PTHR31319//PTHR31319:SF10 - FAMILY NOT NAMED // ZINC FINGER PROTEIN CONSTANS-RELATED; CONSTANS-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTGATATATGTGGTATATGCCAGTGT |
| Internal bar code: | TCGTCCCCCCATCGACGCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.6954 |
| LEAP-Seq n confirming: | 5892 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCACACTCTTCCCGCTT |
| Suggested primer 2: | ACAAACTCACGTCCTGACCC |